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验证数据展示

  • WB analysis using CL750-81805

    WB analysis using CL750-81805

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL750-81805

    WB analysis using CL750-81805

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL750-81805

    FC experiment of HepG2 using CL750-81805

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody (CL750-81805, Clone:1L11)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody

IGF2BP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-81805

产品说明书

CloneNo. 1L11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra)

别名

IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, K-562 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IGF2BP3 Ready-To-Use IHC Kit. IGF2BP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81805-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81805 targets IGF2BP3 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 IGF2BP3 fusion protein Ag6226 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor 2 mRNA binding protein 3
别名 IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3
计算分子量 64 kDa
观测分子量64 kDa
GenBank蛋白编号BC065269
基因名称 IGF2BP3
Gene ID (NCBI) 10643
RRIDAB_3673736
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00425
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 IGF2BP3 antibody CL750-81805Download protocol
FC protocol for CL Plus 750 IGF2BP3 antibody CL750-81805Download protocol
Standard Protocols
Click here to view our Standard Protocols