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CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody

IGF2BP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1L11

Cat no : CL750-81805

Print datasheet

Synonyms

IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3

验证数据展示

  • WB analysis using CL750-81805

    WB analysis using CL750-81805

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL750-81805

    WB analysis using CL750-81805

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL750-81805

    FC experiment of HepG2 using CL750-81805

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody (CL750-81805, Clone:1L11)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, K-562 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IGF2BP3 Ready-To-Use IHC Kit. IGF2BP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81805-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81805 targets IGF2BP3 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen IGF2BP3 fusion protein Ag6226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor 2 mRNA binding protein 3
Synonyms IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3
Calculated Molecular Weight 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC065269
Gene Symbol IGF2BP3
Gene ID (NCBI) 10643
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00425
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 IGF2BP3 antibody CL750-81805Download protocol
FC protocol for CL Plus 750 IGF2BP3 antibody CL750-81805Download protocol
Standard Protocols
Click here to view our Standard Protocols