验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HepG2 cells, K-562 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy IGF2BP3 Ready-To-Use IHC Kit. IGF2BP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81805-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81805 targets IGF2BP3 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | IGF2BP3 fusion protein Ag6226 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | insulin-like growth factor 2 mRNA binding protein 3 |
| Synonyms | IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3 |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC065269 |
| Gene Symbol | IGF2BP3 |
| Gene ID (NCBI) | 10643 |
| RRID | AB_3673736 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00425 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 IGF2BP3 antibody CL750-81805 | Download protocol |
| FC protocol for CL Plus 750 IGF2BP3 antibody CL750-81805 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |