验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy IGF2BP3 Ready-To-Use IHC Kit. IGF2BP3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66526 targets IGF2BP3 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | IGF2BP3 fusion protein Ag6349 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | insulin-like growth factor 2 mRNA binding protein 3 |
| 别名 | DKFZp686F1078, hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC065269 |
| 基因名称 | IGF2BP3 |
| Gene ID (NCBI) | 10643 |
| RRID | AB_2934475 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00425 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IGF2BP3 antibody CL488-66526 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |