• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66644-1-Ig

    WB analysis using 66644-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66644-1-Ig (IGFBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 66644-1-Ig

    IF Staining of A549 using 66644-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IGFBP2 antibody (66644-1-Ig, Clone: 2F10D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

IGFBP2 Monoclonal antibody

IGFBP2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66644-1-Ig
CloneNo. 2F10D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

IBP-2, IBP2, IBP 2, BP2, BP 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, T-27D cells, MCF-7 cells, PC-3 cells, NCCIT cells, 4T1 cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66644-1-Ig targets IGFBP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IGFBP2 fusion protein Ag8421 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IGF binding protein 2
Synonyms IBP-2, IBP2, IBP 2, BP2, BP 2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004312
Gene Symbol IGFBP2
Gene ID (NCBI) 3485
RRIDAB_2882002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.

实验方案

Product Specific Protocols
WB protocol for IGFBP2 antibody 66644-1-IgDownload protocol
IF protocol for IGFBP2 antibody 66644-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols