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验证数据展示

  • IF Staining of MCF-7 using CL488-66644

    IF Staining of MCF-7 using CL488-66644

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 IGFBP2 antibody (CL488-66644, Clone: 2F10D1 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-66644

    FC experiment of MCF-7 using CL488-66644

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IGFBP2 (CL488-66644, Clone:2F10D1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated IGFBP2 Monoclonal antibody

IGFBP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66644
CloneNo. 2F10D1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66644-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66644 targets IGFBP2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, Mouse, rat
Immunogen IGFBP2 fusion protein Ag8421 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IGF binding protein 2
Synonyms BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004312
Gene Symbol IGFBP2
Gene ID (NCBI) 3485
RRIDAB_2934485
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18065
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IGFBP2 antibody CL488-66644Download protocol
FC protocol for CL Plus 488 IGFBP2 antibody CL488-66644Download protocol
Standard Protocols
Click here to view our Standard Protocols