CoraLite® Plus 488-conjugated IGFBP2 Monoclonal antibody
IGFBP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F10D1
验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66644-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66644 targets IGFBP2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | Human, Mouse, rat |
Immunogen | IGFBP2 fusion protein Ag8421 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | IGF binding protein 2 |
Synonyms | BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2 |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC004312 |
Gene Symbol | IGFBP2 |
Gene ID (NCBI) | 3485 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P18065 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 IGFBP2 antibody CL488-66644 | Download protocol |
FC protocol for CL Plus 488 IGFBP2 antibody CL488-66644 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |