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验证数据展示

  • IF Staining of MCF-7 using CL488-66644

    IF Staining of MCF-7 using CL488-66644

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 IGFBP2 antibody (CL488-66644, Clone: 2F10D1 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-66644

    FC experiment of MCF-7 using CL488-66644

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IGFBP2 (CL488-66644, Clone:2F10D1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated IGFBP2 Monoclonal antibody

IGFBP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66644

产品说明书

CloneNo. 2F10D1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, rat

应用

IF/ICC, FC (Intra)

别名

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66644-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66644 targets IGFBP2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, Mouse, rat
免疫原 IGFBP2 fusion protein Ag8421 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IGF binding protein 2
别名 BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004312
基因名称 IGFBP2
Gene ID (NCBI) 3485
RRIDAB_2934485
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18065
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IGFBP2 antibody CL488-66644Download protocol
FC protocol for CL Plus 488 IGFBP2 antibody CL488-66644Download protocol
Standard Protocols
Click here to view our Standard Protocols