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IGFBP3 Monoclonal antibody, PBS Only

IGFBP3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12A11

Cat no : 68402-1-PBS

Print datasheet

Synonyms

IGF binding protein 3, IBP-3, IBP3, IBP 3, BP 53

验证数据展示

  • WB analysis of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    WB analysis of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

  • WB analysis using 68402-1-Ig (same clone as 68402-1-PBS)

    WB analysis using 68402-1-Ig (same clone as 68402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    IHC staining of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68402-1-Ig (IGFBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGFBP3 antibody included
Planning an IHC experiment? We recommend our IHCeasy IGFBP3 Ready-To-Use IHC Kit. IGFBP3 primary antibody included.

产品信息

68402-1-PBS targets IGFBP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen IGFBP3 fusion protein Ag20351 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name insulin-like growth factor binding protein 3
Synonyms IGF binding protein 3, IBP-3, IBP3, IBP 3, BP 53
Calculated Molecular Weight 32 kDa
Observed Molecular Weight39-45 kDa
GenBank Accession NumberBC000013
Gene Symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP17936
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)