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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    WB analysis of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

  • WB analysis using 68402-1-Ig (same clone as 68402-1-PBS)

    WB analysis using 68402-1-Ig (same clone as 68402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    IHC staining of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68402-1-Ig (IGFBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGFBP3 antibody included

IGFBP3 Monoclonal antibody, PBS Only

IGFBP3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68402-1-PBS

产品说明书

CloneNo. 1A12A11

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

IGF binding protein 3, IBP-3, IBP3, IBP 3, BP 53

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IGFBP3 Ready-To-Use IHC Kit. IGFBP3 primary antibody included.

产品信息

68402-1-PBS targets IGFBP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 IGFBP3 fusion protein Ag20351 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 insulin-like growth factor binding protein 3
别名 IGF binding protein 3, IBP-3, IBP3, IBP 3, BP 53
计算分子量 32 kDa
观测分子量39-45 kDa
GenBank蛋白编号BC000013
基因名称 IGFBP3
Gene ID (NCBI) 3486
偶联类型 Unconjugated
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDP17936
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)