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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83200-3-RR (IGFBP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug IGFBP4 Recombinant antibody (83200-3-RR, Clone:230543F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83200-3-RR

    Affinity and Kinetic Characterization of 83200-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83200-3-RR against Human IGFBP4 were performed. The affinity constant is 0.776 nM.

IGFBP4 Recombinant antibody, PBS Only

IGFBP4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 83200-3-PBS

产品说明书

CloneNo. 230543F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83200-3-PBS targets IGFBP4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor binding protein 4
别名 IBP 4, HT29 IGFBP, Deb2, BP4, BP 4
计算分子量28 kDa
观测分子量 28-40 kDa
GenBank蛋白编号NM_001552.3
基因名称 IGFBP4
Gene ID (NCBI) 3487
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP22692-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).