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IGFBP4 Recombinant antibody, PBS Only

IGFBP4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230543F8

Cat no : 83200-3-PBS

Print datasheet

Synonyms

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

验证数据展示

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83200-3-RR (IGFBP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug IGFBP4 Recombinant antibody (83200-3-RR, Clone:230543F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83200-3-RR

    Affinity and Kinetic Characterization of 83200-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83200-3-RR against Human IGFBP4 were performed. The affinity constant is 0.776 nM.

产品信息

The immunogen of 83200-3-PBS is IGFBP4 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor binding protein 4
Synonyms IBP 4, HT29 IGFBP, Deb2, BP4, BP 4
Calculated Molecular Weight28 kDa
Observed Molecular Weight 28-40 kDa
GenBank Accession NumberNM_001552.3
Gene Symbol IGFBP4
Gene ID (NCBI) 3487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22692-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).