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IGFBP4 Recombinant antibody, PBS Only

IGFBP4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230543F8

Cat no : 83200-3-PBS

Print datasheet

Synonyms

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

验证数据展示

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83200-3-RR (IGFBP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug IGFBP4 Recombinant antibody (83200-3-RR, Clone:230543F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83200-3-RR

    Affinity and Kinetic Characterization of 83200-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83200-3-RR against Human IGFBP4 were performed. The affinity constant is 0.776 nM.

产品信息

83200-3-PBS targets IGFBP4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor binding protein 4
Synonyms IBP 4, HT29 IGFBP, Deb2, BP4, BP 4
Calculated Molecular Weight28 kDa
Observed Molecular Weight 28-40 kDa
GenBank Accession NumberNM_001552.3
Gene Symbol IGFBP4
Gene ID (NCBI) 3487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22692-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).