IGFBP4 Recombinant antibody
IGFBP4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230543F8
验证数据展示
经过测试的应用
Positive WB detected in | MDA-MB-231 cells, K-562 cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83200-3-RR targets IGFBP4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | Fusion Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | insulin-like growth factor binding protein 4 |
Synonyms | IBP 4, HT29 IGFBP, Deb2, BP4, BP 4 |
Calculated Molecular Weight | 28 kDa |
Observed Molecular Weight | 28-40 kDa |
GenBank Accession Number | NM_001552.3 |
Gene Symbol | IGFBP4 |
Gene ID (NCBI) | 3487 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P22692-1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IGFBP4 antibody 83200-3-RR | Download protocol |
IHC protocol for IGFBP4 antibody 83200-3-RR | Download protocol |
FC protocol for IGFBP4 antibody 83200-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |