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IGFBP6 Recombinant antibody

IGFBP6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241724H5

Cat no : 84364-5-RR

Print datasheet

Synonyms

IGF-binding protein 6, IGF binding protein 6, IBP-6, IBP6, IBP 6

验证数据展示

  • WB analysis of human testis using 84364-5-RR

    WB analysis of human testis using 84364-5-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 84364-5-RR (IGFBP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84364-5-RR

    Affinity and Kinetic Characterization of 84364-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84364-5-RR against Human IGFBP6 were performed. The affinity constant is 0.259 nM.

经过测试的应用

Positive WB detected inhuman testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84364-5-RR targets IGFBP6 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor binding protein 6
Synonyms IGF-binding protein 6, IGF binding protein 6, IBP-6, IBP6, IBP 6
Calculated Molecular Weight 25 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC011708
Gene Symbol IGFBP6
Gene ID (NCBI) 3489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24592
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factor (IGF) binding protein (IGFBP6), a 240 amino acid protein, contains an IGFBP N-terminal domain and a thyroglobulin type-1 domain. It modulates the activity of IGF and shows independent effects of IGF, such as growth inhibition and apoptosis. It can decrease the proliferation and survival of cancer cells such as lung cancer cells and naso-pharyngeal cancer cells. IGFBP-6 is distinctive for its 50-fold higher binding affinity for IGF-II over IGF-I and this specificity makes it an attractive potential therapeutic candidate for IGF-II-dependent pediatric malignancies such as rhabdomyosarcoma (RMS). In addition, it was found that IGFBP6 can promote the migration of RMS cells in an IGF-independent manner, and MAPK pathways were involved in this process. Further study reported that IGFBP6 is one of most highly expressed proteins in varicose vein tissues and is involved in the proliferation of vascular smooth muscle cells (VSMCs), which may provide insights into the underlying pathogenesis of varicose vein.

实验方案

Product Specific Protocols
WB protocol for IGFBP6 antibody 84364-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols