验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67538-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67538 targets IGHD in IF-P applications and shows reactivity with Human samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human |
| 免疫原 | IGHD fusion protein Ag9614 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant delta |
| 别名 | FLJ00382, FLJ46727, IGHD |
| 计算分子量 | 573 aa, 63 kDa |
| 观测分子量 | 55-70 kDa |
| GenBank蛋白编号 | BC021276 |
| 基因名称 | IGHD |
| Gene ID (NCBI) | 3495 |
| RRID | AB_2919496 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IGHD antibody CL488-67538 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |