验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16402-1-AP targets human IgG heavy chain in WB, IHC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP, RIP |
| 经测试反应性 | human |
| 免疫原 | human IgG heavy chain fusion protein Ag9519 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant gamma 4 (G4m marker) |
| 别名 | Human IgG4, IgG Fc, IGHG4, IGHG, IgG4 |
| 计算分子量 | 473 aa, 52 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC025985 |
| 基因名称 | IGHG4 |
| Gene ID (NCBI) | 3503 |
| RRID | AB_2878254 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01861 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for human IgG heavy chain antibody 16402-1-AP | Download protocol |
| IHC protocol for human IgG heavy chain antibody 16402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia. | ||
Neuropathol Appl Neurobiol Plasma total prion protein as a potential biomarker for neurodegenerative dementia: diagnostic accuracy in the spectrum of prion diseases. | ||
Mol Oncol Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer. | ||
Cancer Gene Ther miR-451a was selectively sorted into exosomes and promoted the progression of esophageal squamous cell carcinoma through CAB39 | ||
J Dent Sci Circ_0004491 stimulates guanine nucleotide-binding protein alpha subunit to inhibit the malignant progression of oral squamous cell carcinoma by sponging miR-2278 | ||
Oncol Rep Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells. |
