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验证数据展示

  • WB analysis using 68531-1-Ig

    WB analysis using 68531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68531-1-Ig (IKBKE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68531-1-Ig

    WB analysis using 68531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68531-1-Ig (IKBKE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IKBKE Monoclonal antibody

IKBKE Monoclonal Antibody for WB, ELISA
Cat No. 68531-1-Ig

产品说明书

CloneNo. 3E4H7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

I kappa B kinase epsilon, IKBKE, IKK E, IKK epsilon, IKK i, IKKE, IKKI, Inducible I kappa B kinase, KIAA0151

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSK-BR-3 cells, Jurkat cells, MCF-7 cells, HeLa cells, Raji cells, Ramos cells, Daudi cells, HL-60 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68531-1-Ig targets IKBKE in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 IKBKE fusion protein Ag30374 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
别名 I kappa B kinase epsilon, IKBKE, IKK E, IKK epsilon, IKK i, IKKE, IKKI, Inducible I kappa B kinase, KIAA0151
计算分子量 716 aa, 80 kDa
观测分子量80 kDa
GenBank蛋白编号BC107812
基因名称 IKBKE
Gene ID (NCBI) 9641
RRIDAB_3085239
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14164
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IKBKE is a serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. It is also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling.

实验方案

Product Specific Protocols
WB protocol for IKBKE antibody 68531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols