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IKKA Recombinant antibody

IKKA Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241579A2

Cat no : 84372-4-RR

Print datasheet

Synonyms

CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha

验证数据展示

  • WB analysis using 84372-4-RR

    WB analysis using 84372-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84372-4-RR (IKKA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84372-4-RR

    FC experiment of HepG2 using 84372-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant antibody (84372-4-RR, Clone:241579A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84372-4-RR

    Affinity and Kinetic Characterization of 84372-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84372-4-RR against Human IKKA were performed. The affinity constant is 19.5 nM.

经过测试的应用

Positive WB detected inJurkat cells, Daudi cells, HeLa cells, HepG2 cells, RAW 264.7 cells, C6 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84372-4-RR is IKKA Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IKKA fusion protein Ag26573 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name conserved helix-loop-helix ubiquitous kinase
Synonyms CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha
Calculated Molecular Weight 85 kDa
Observed Molecular Weight85 kDa
GenBank Accession NumberNM_001278
Gene Symbol IKK Alpha
Gene ID (NCBI) 1147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IKKA antibody 84372-4-RRDownload protocol
FC protocol for IKKA antibody 84372-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols