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验证数据展示

  • WB analysis using 84372-4-RR

    WB analysis using 84372-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84372-4-RR (IKKA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84372-4-RR

    FC experiment of HepG2 using 84372-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant antibody (84372-4-RR, Clone:241579A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84372-4-RR

    Affinity and Kinetic Characterization of 84372-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84372-4-RR against Human IKKA were performed. The affinity constant is 19.5 nM.

IKKA Recombinant antibody

IKKA Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84372-4-RR

产品说明书

CloneNo. 241579A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Daudi cells, HeLa cells, HepG2 cells, RAW 264.7 cells, C6 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84372-4-RR targets IKKA in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IKKA fusion protein Ag26573 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 conserved helix-loop-helix ubiquitous kinase
别名 CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha
计算分子量 85 kDa
观测分子量85 kDa
GenBank蛋白编号NM_001278
基因名称 IKK Alpha
Gene ID (NCBI) 1147
RRIDAB_3671907
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15111
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IKKA antibody 84372-4-RRDownload protocol
FC protocol for IKKA antibody 84372-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols