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IL-1 beta Recombinant antibody

IL-1 beta Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240902G6

Cat no : 82696-17-RR

Print datasheet

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

验证数据展示

  • WB analysis using 82696-17-RR

    WB analysis using 82696-17-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 82696-17-RR

    WB analysis of THP-1 using 82696-17-RR

    LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82696-17-RR

    Affinity and Kinetic Characterization of 82696-17-RR

    Biolayer interferometry (BLl) kinetic assays of 82696-17-RR against Human IL-1 beta were performed. The affinity constant is 60.2 nM.

经过测试的应用

Positive WB detected inA431 cells, LPS and Brefeldin A treated THP-1 cells, HT-1080 cells, U-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 82696-17-RR is IL-1 beta Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 1, beta
Synonyms IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin
Calculated Molecular Weight 269 aa, 31 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC008678
Gene Symbol IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01584
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to the activation of numerous downstream targets.

实验方案

Product Specific Protocols
WB protocol for IL-1 beta antibody 82696-17-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols