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Anti-Human IL-1 beta Rabbit Recombinant Antibody

IL-1 beta Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240902A3

Cat no : 98142-1-RR

Print datasheet

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

验证数据展示

  • FC experiment of human PBMCs using 98142-1-RR

    FC experiment of human PBMCs using 98142-1-RR

    1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • FC experiment of human PBMCs using 98142-1-RR

    FC experiment of human PBMCs using 98142-1-RR

    1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • Affinity and Kinetic Characterization of 98142-1-RR

    Affinity and Kinetic Characterization of 98142-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98142-1-RR against Human IL-1 beta were performed. The affinity constant is 4.98 nM.

经过测试的应用

Positive FC (Intra) detected inIFN gamma, LPS and Brefeldin A treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98142-1-RR is IL-1 beta Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 1, beta
Synonyms IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin
Calculated Molecular Weight 269 aa, 31 kDa
GenBank Accession NumberBC008678
Gene Symbol IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01584
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

实验方案

Product Specific Protocols
FC protocol for IL-1 beta antibody 98142-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols