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Species Reactivity
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 98142-1-RR

    FC experiment of human PBMCs using 98142-1-RR

    1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • FC experiment of human PBMCs using 98142-1-RR

    FC experiment of human PBMCs using 98142-1-RR

    1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • Affinity and Kinetic Characterization of 98142-1-RR

    Affinity and Kinetic Characterization of 98142-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98142-1-RR against Human IL-1 beta were performed. The affinity constant is 4.98 nM.

Anti-Human IL-1 beta Rabbit Recombinant Antibody

IL-1 beta Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98142-1-RR

产品说明书

CloneNo. 240902A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inIFN gamma, LPS and Brefeldin A treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98142-1-RR targets IL-1 beta in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 1, beta
别名 IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin
计算分子量 269 aa, 31 kDa
GenBank蛋白编号BC008678
基因名称 IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
RRIDAB_3672288
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01584
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

实验方案

Product Specific Protocols
FC protocol for IL-1 beta antibody 98142-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols