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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 65286-1-Ig (same clone as 65286-1-PBS)

    FC experiment of human PBMCs using 65286-1-Ig (same clone as 65286-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) or Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65286-1-Ig. This data was developed using the same antibody clone with 65286-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65286-1-Ig (same clone as 65286-1-PBS)

    FC experiment of human PBMCs using 65286-1-Ig (same clone as 65286-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) (red) or Mouse IgG2b Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65286-1-Ig. This data was developed using the same antibody clone with 65286-1-PBS in a different storage buffer formulation.

Anti-Human IL-10 (7B8G3), PBS Only

IL-10 Monoclonal Antibody for FC
Cat No. 65286-1-PBS

Print datasheet

CloneNo. 7B8G3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Synonyms

IL-10,IL10, 7B8G3, CSIF, IL 10, IL10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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65286-1-Ig

产品信息

65286-1-PBS targets IL-10 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen IL-10 fusion protein HZ-1145 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 10
Synonyms IL-10,IL10, 7B8G3, CSIF, IL 10, IL10
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC104252
Gene Symbol IL-10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.