验证数据展示
经过测试的应用
Positive FC (Intra) detected in | LPS and Brefeldin A treated human PBMCs |
Positive FC detected in | LPS and Brefeldin A treated human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65286 targets IL-10 in FC (Intra) applications and shows reactivity with Human samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | Human |
免疫原 | IL-10 fusion protein HZ-1145 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | interleukin 10 |
别名 | IL-10, IL-10,IL10, IL10A, interleukin 10, Interleukin-10, TGIF, 7B8G3, CSIF, IL 10, IL10 |
计算分子量 | 21 kDa |
GenBank蛋白编号 | BC104252 |
基因名称 | IL-10 |
Gene ID (NCBI) | 3586 |
RRID | AB_3084819 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 IL-10 antibody CL647-65286 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |