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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL647-65286

    FC experiment of human PBMCs using CL647-65286

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-10 (CL647-65286, Clone:7B8G3) or Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65286

    FC experiment of human PBMCs using CL647-65286

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-10 (CL647-65286, Clone:7B8G3) (red) or Mouse IgG2b Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-10 (CL647-65286, Clone:7B8G3) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 647 Anti-Human IL-10 (7B8G3)

IL-10 Monoclonal Antibody for FC (Intra)
Cat No. CL647-65286

Print datasheet

CloneNo. 7B8G3

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra)

Synonyms

IL-10, IL-10,IL10, IL10A, interleukin 10, Interleukin-10, TGIF, 7B8G3, CSIF, IL 10, IL10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65286-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65286 targets IL-10 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen IL-10 fusion protein HZ-1145 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 10
Synonyms IL-10, IL-10,IL10, IL10A, interleukin 10, Interleukin-10, TGIF, 7B8G3, CSIF, IL 10, IL10
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC104252
Gene Symbol IL-10
Gene ID (NCBI) 3586
RRIDAB_3084819
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IL-10 antibody CL647-65286Download protocol
Standard Protocols
Click here to view our Standard Protocols