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验证数据展示

  • FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite ® Plus 750 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite ® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unstimulated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

Anti-Human IL-12 (1E12H6), PBS Only

IL-12 Monoclonal Antibody for FC (Intra)
Cat No. 65277-1-PBS

产品说明书

CloneNo. 1E12H6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

65277-1-PBS targets IL-12 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 IL-12 fusion protein HZ-1256 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
别名 1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A
计算分子量 25 kDa
GenBank蛋白编号BC104982
基因名称 IL-12A
Gene ID (NCBI) 3592
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件-20°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).