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Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody, PBS Only

IL-12/IL-23 p40 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241600F8

Cat no : 98277-1-PBS

Print datasheet

Synonyms

IL12B, IL 12B, IL 12 subunit p40, IL 12 P40, Cytotoxic lymphocyte maturation factor 40 kDa subunit

验证数据展示

  • FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98277-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL12B Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98277-1-PBS in a different storage buffer formulation.

产品信息

98277-1-PBS targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Synonyms IL12B, IL 12B, IL 12 subunit p40, IL 12 P40, Cytotoxic lymphocyte maturation factor 40 kDa subunit
Calculated Molecular Weight 328 aa, 37 kDa
GenBank Accession NumberBC067498
Gene Symbol IL12B
Gene ID (NCBI) 3593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP29460
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).