验证数据展示
经过测试的应用
| Positive WB detected in | PMA, Ionomycin and Brefeldin A treated EL-4 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82905-1-RR targets IL-17A in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | mouse |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | interleukin 17A |
| Synonyms | Il17a, Interleukin17A, interleukin 17A, Il17, IL 17A |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 14 kDa, 17 kDa |
| GenBank Accession Number | NM-010552 |
| Gene Symbol | Il17a |
| Gene ID (NCBI) | 16171 |
| RRID | AB_3670633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q62386 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-17A antibody 82905-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |