验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 66143-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66143 targets IL-17D in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | IL-17D fusion protein Ag6762 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 17D |
| 别名 | IL17D, interleukin 17D, IL-27, IL27, IL 27 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC036243 |
| 基因名称 | IL-17D |
| Gene ID (NCBI) | 53342 |
| RRID | AB_3084213 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8TAD2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL17D is a secreted cytokine with homology to the IL-17 family of proteins. IL17D is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas. The treatment of endothelial cells with IL17D has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by IL17D was found to be NF-kappa B-dependent.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IL-17D antibody CL488-66143 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |