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验证数据展示

  • WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

    WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    WB result of Pre-IL18 antibody (60070-1-Ig, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells.. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IL18 antibody included
  • IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IL18 antibody included
  • WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

    WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    IF Staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    IF Staining of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    FC experiment of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug IL-18 Monoclonal antibody (60070-1-Ig, Clone:5C6F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51140-3

    Cytometric bead array standard curve of MP51140-3

    Cytometric bead array standard curve of MP51140-3, IL-18 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60070-1-PBS. Detection antibody: 60070-2-PBS. Standard:Ag1063. Range: 0.098-100 ng/mL.

  • Featured Product
  • KD/KO验证

IL-18 Monoclonal antibody, PBS Only (Capture)

IL-18 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 60070-1-PBS

产品说明书

CloneNo. 5C6F8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

别名

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IL18 Ready-To-Use IHC Kit. IL18 primary antibody included.

产品信息

60070-1-PBS targets IL-18 as part of a matched antibody pair:

MP51140-3: 60070-1-PBS capture and 60070-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 IL-18 fusion protein Ag1063 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 IL-18
别名 IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma
计算分子量 193 aa, 22 kDa
观测分子量 24 kDa
GenBank蛋白编号BC007461
基因名称 IL-18
Gene ID (NCBI) 3606
RRIDAB_2280158
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14116
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.