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IL-18 Recombinant antibody, PBS Only

IL-18 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H14

Cat no : 82907-1-PBS

Print datasheet

Synonyms

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

验证数据展示

  • WB analysis using 82907-1-RR (same clone as 82907-1-PBS)

    WB analysis using 82907-1-RR (same clone as 82907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82907-1-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

    IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IL18 antibody included
  • IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

    IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IL18 antibody included
  • FC experiment of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

    FC experiment of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IL-18 Recombinant antibody (82907-1-RR, Clone:1H14) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

    IF Staining of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (82907-1-RR, Clone: 1H14 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82907-1-RR

    Affinity and Kinetic Characterization of 82907-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82907-1-RR against Human IL-18 were performed. The affinity constant is 0.769 nM.

Planning an IHC experiment? We recommend our IHCeasy IL18 Ready-To-Use IHC Kit. IL18 primary antibody included.

产品信息

82907-1-PBS targets IL-18 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen IL-18 fusion protein Ag1063 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IL-18
Synonyms IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma
Calculated Molecular Weight 193 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC007461
Gene Symbol IL-18
Gene ID (NCBI) 3606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.