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IL-18 Recombinant antibody

IL-18 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H14

Cat no : 82907-1-RR

Print datasheet

Synonyms

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

验证数据展示

  • WB analysis using 82907-1-RR

    WB analysis using 82907-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82907-1-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 82907-1-RR

    IHC staining of human tonsillitis using 82907-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IL18 antibody included
  • IHC staining of human tonsillitis using 82907-1-RR

    IHC staining of human tonsillitis using 82907-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IL18 antibody included
  • FC experiment of HeLa using 82907-1-RR

    FC experiment of HeLa using 82907-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IL-18 Recombinant antibody (82907-1-RR, Clone:1H14) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 82907-1-RR

    IF Staining of HeLa using 82907-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (82907-1-RR, Clone: 1H14 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82907-1-RR

    Affinity and Kinetic Characterization of 82907-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82907-1-RR against Human IL-18 were performed. The affinity constant is 0.769 nM.

经过测试的应用

Positive WB detected inA549 cells, U2OS cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IL18 Ready-To-Use IHC Kit. IL18 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82907-1-RR targets IL-18 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen IL-18 fusion protein Ag1063 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IL-18
Synonyms IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma
Calculated Molecular Weight 193 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC007461
Gene Symbol IL-18
Gene ID (NCBI) 3606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.

实验方案

Product Specific Protocols
WB protocol for IL-18 antibody 82907-1-RRDownload protocol
IHC protocol for IL-18 antibody 82907-1-RRDownload protocol
IF protocol for IL-18 antibody 82907-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols