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验证数据展示

  • IF Staining of HeLa using CL488-60070

    IF Staining of HeLa using CL488-60070

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 IL-18 antibody (CL488-60070, Clone: 5C6F8 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-60070

    FC experiment of HeLa using CL488-60070

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-18 (CL488-60070, Clone:5C6F8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO验证

CoraLite® Plus 488-conjugated IL-18 Monoclonal antibody

IL-18 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-60070

产品说明书

CloneNo. 5C6F8

宿主/亚型

Mouse / IgG2b

种属反应性

human, hamster

应用

IF/ICC, FC (Intra)

别名

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IL18 Ready-To-Use IHC Kit. IL18 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60070-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60070 targets IL-18 in IF/ICC, FC (Intra) applications and shows reactivity with human, hamster samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, hamster
免疫原 IL-18 fusion protein Ag1063 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 IL-18
别名 IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma
计算分子量 193 aa, 22 kDa
观测分子量 24 kDa
GenBank蛋白编号BC007461
基因名称 IL-18
Gene ID (NCBI) 3606
RRIDAB_3084138
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14116
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IL-18 antibody CL488-60070Download protocol
FC protocol for CL Plus 488 IL-18 antibody CL488-60070Download protocol
Standard Protocols
Click here to view our Standard Protocols