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CoraLite® Plus 488-conjugated IL-1F10 Monoclonal antibody

IL-1F10 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5B2F5

Cat no : CL488-60319

Print datasheet

Synonyms

IL1F10, IL 1F10, IL 1 theta, FKSG75, FIL1T

验证数据展示

  • FC experiment of U-937 using CL488-60319

    FC experiment of U-937 using CL488-60319

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1F10 (CL488-60319, Clone:5B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inU-937 cells
For other applications, we recommend the unconjugated version of this antibody, 60319-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60319 targets IL-1F10 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen IL-1F10 fusion protein Ag17250 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 1 family, member 10 (theta)
Synonyms IL1F10, IL 1F10, IL 1 theta, FKSG75, FIL1T
Calculated Molecular Weight 152 aa, 17 kDa
GenBank Accession NumberBC103966
Gene Symbol IL1F10
Gene ID (NCBI) 84639
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WWZ1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-1F10 antibody CL488-60319Download protocol
Standard Protocols
Click here to view our Standard Protocols