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验证数据展示

  • FC experiment of U-937 using CL488-60319

    FC experiment of U-937 using CL488-60319

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1F10 (CL488-60319, Clone:5B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated IL-1F10 Monoclonal antibody

IL-1F10 Monoclonal Antibody for FC (Intra)
Cat No. CL488-60319

产品说明书

CloneNo. 5B2F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

IL1F10, IL 1F10, IL 1 theta, FKSG75, FIL1T

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inU-937 cells
For other applications, we recommend the unconjugated version of this antibody, 60319-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60319 targets IL-1F10 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 IL-1F10 fusion protein Ag17250 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 1 family, member 10 (theta)
别名 IL1F10, IL 1F10, IL 1 theta, FKSG75, FIL1T
计算分子量 152 aa, 17 kDa
GenBank蛋白编号BC103966
基因名称 IL1F10
Gene ID (NCBI) 84639
RRIDAB_3084147
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WWZ1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-1F10 antibody CL488-60319Download protocol
Standard Protocols
Click here to view our Standard Protocols