IL-2 Polyclonal antibody
IL-2 Polyclonal Antibody for ELISA, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
rat
Applications
ELISA, FC (Intra)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA and ionomycin treated rat splenocytes cells |
| Positive FC detected in | PMA and ionomycin treated rat splenocytes cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30199-1-AP targets IL-2 in ELISA, FC (Intra) applications and shows reactivity with rat samples.
| Tested Applications | ELISA, FC (Intra) Application Description |
| Tested Reactivity | rat |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interleukin 2 |
| Synonyms | Il2, IL-2, interleukin 2 |
| Calculated Molecular Weight | 18 kDa |
| GenBank Accession Number | NM_053836 |
| Gene Symbol | Il2 |
| Gene ID (NCBI) | 116562 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17108 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-2 antibody 30199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
