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Anti-Mouse IL-2 Rabbit Recombinant Antibody, PBS Only

IL-2 Uni-rAbTM Recombinant Antibody for FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240358A2

Cat no : 98055-1-PBS

Print datasheet

Synonyms

Il2, T-cell growth factor, Interleukin-2, interleukin 2, Il 2

验证数据展示

  • FC experiment of mouse splenocytes using 98055-1-RR (same clone as 98055-1-PBS)

    FC experiment of mouse splenocytes using 98055-1-RR (same clone as 98055-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-2 Rabbit Recombinant Antibody (98055-1-RR, Clone: 240358A2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98055-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00533-3

    Sandwich ELISA standard curve of MP00533-3

    Sandwich ELISA standard curve of MP00533-3, mouse IL-2 Recombinant Matched Antibody Pair - PBS only. 98055-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0762. 83547-1-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

  • Sample test of MP00533-3

    Sample test of MP00533-3

    EL-4 mouse lymphoblast cells (1 x 10^6  cells/mL) were cultured for 3 days in DMEM supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL PHA and 10 ng/mL PMA. The mean mouse IL-2 concentration was determined to be 16.1 pg/mL in unstimulated EL-4, 309.9 ng/mL in PHA and PMA stimulated EL-4 supernatant.

  • Sample test of MP00533-3

    Sample test of MP00533-3

    Serum of sixteen mice was measured. were measured. The mouse IL-2 concentration was determined to be 2.27 pg/mL with a range of 1.1-79.9 pg/mL.

  • Affinity and Kinetic Characterization of 98055-1-RR

    Affinity and Kinetic Characterization of 98055-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98055-1-RR against Mouse IL-2 were performed. The affinity constant is 0.229 nM.

产品信息

98055-1-PBS targets IL-2 as part of a matched antibody pair:

MP00533-3: 98055-1-PBS capture and 83547-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2
Synonyms Il2, T-cell growth factor, Interleukin-2, interleukin 2, Il 2
Calculated Molecular Weight 19 kDa
GenBank Accession Number NM_008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04351
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).