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Anti-Mouse IL-2 Rabbit Recombinant Antibody

IL-2 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240358A2

Cat no : 98055-1-RR

Print datasheet

Synonyms

Il2, T-cell growth factor, Interleukin-2, interleukin 2, Il 2

验证数据展示

  • FC experiment of mouse splenocytes using 98055-1-RR

    FC experiment of mouse splenocytes using 98055-1-RR

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-2 Rabbit Recombinant Antibody (98055-1-RR, Clone: 240358A2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98055-1-RR

    Affinity and Kinetic Characterization of 98055-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98055-1-RR against Mouse IL-2 were performed. The affinity constant is 0.229 nM.

经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98055-1-RR targets IL-2 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2
Synonyms Il2, T-cell growth factor, Interleukin-2, interleukin 2, Il 2
Calculated Molecular Weight 19 kDa
GenBank Accession Number NM_008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04351
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

实验方案

Product Specific Protocols
FC protocol for IL-2 antibody 98055-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols