• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 66142-1-Ig (same clone as 66142-1-PBS)

    WB analysis of Jurkat using 66142-1-Ig (same clone as 66142-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66142-1-Ig (IL-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

    IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

    IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

    IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 66142-1-Ig (same clone as 66142-1-PBS)

    FC experiment of Ramos using 66142-1-Ig (same clone as 66142-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human IL-4 (66142-1-Ig, Clone:1G2C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IL-4 Monoclonal antibody, PBS Only

IL-4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 66142-1-PBS

产品说明书

CloneNo. 1G2C5

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra, PS1TP5BP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66142-1-PBS targets IL-4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, pig
免疫原 IL-4 fusion protein Ag9940 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 4
别名 B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra, PS1TP5BP1
计算分子量 153 aa, 17 kDa
观测分子量 18 kDa
GenBank蛋白编号BC070123
基因名称 IL-4
Gene ID (NCBI) 3565
RRIDAB_2881539
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05112
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.