• Featured Product
  • KD/KO Validated

IL-4 Monoclonal antibody

IL-4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (3)

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1G2C5

Cat no : 66142-1-Ig

Print datasheet

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, PS1TP5BP1



经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive FC (Intra) detected inRamos cells
Positive FC detected inRamos cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66142-1-Ig targets IL-4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, ELISA
Tested Reactivity human, pig
Cited Reactivityhuman, mouse, rat, pig, bovine
Immunogen IL-4 fusion protein Ag9940 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin 4
Synonyms B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, PS1TP5BP1
Calculated Molecular Weight 153 aa, 17 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC070123
Gene Symbol IL-4
Gene ID (NCBI) 3565
RRIDAB_2881539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

实验方案

Product Specific Protocols
WB protocol for IL-4 antibody 66142-1-IgDownload protocol
IHC protocol for IL-4 antibody 66142-1-IgDownload protocol
IF protocol for IL-4 antibody 66142-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Adv Healthc Mater

One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing.

Authors - Shen Guo
ratWB

Aging (Albany NY)

Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis.

Authors - Zhenglan Han
ratWB

Transl Stroke Res

Beneficial Effects of Theta-Burst Transcranial Magnetic Stimulation on Stroke Injury via Improving Neuronal Microenvironment and Mitochondrial Integrity.

Authors - Xuemei Zong

Int Immunopharmacol

Favipiravir ameliorates bleomycin-induced pulmonary fibrosis by reprogramming M1/M2 macrophage polarization

Authors - Ruiqin Zhang
pigWB

Front Cell Dev Biol

Intravenous AAV9 administration results in safe and widespread distribution of transgene in the brain of mini-pig

Authors - Yingqi Lin
humanIHC

Thyroid

A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE

Authors - YuanPing Hai