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- KD/KO Validated
IL-4 Monoclonal antibody
IL-4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig and More (3)
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1G2C5
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue |
Positive FC (Intra) detected in | Ramos cells |
Positive FC detected in | Ramos cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66142-1-Ig targets IL-4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF, ELISA |
Tested Reactivity | human, pig |
Cited Reactivity | human, mouse, rat, pig, bovine |
Immunogen | IL-4 fusion protein Ag9940 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | interleukin 4 |
Synonyms | B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, PS1TP5BP1 |
Calculated Molecular Weight | 153 aa, 17 kDa |
Observed Molecular Weight | 18 kDa |
GenBank Accession Number | BC070123 |
Gene Symbol | IL-4 |
Gene ID (NCBI) | 3565 |
RRID | AB_2881539 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P05112 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for IL-4 antibody 66142-1-Ig | Download protocol |
IHC protocol for IL-4 antibody 66142-1-Ig | Download protocol |
IF protocol for IL-4 antibody 66142-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
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Int Immunopharmacol Favipiravir ameliorates bleomycin-induced pulmonary fibrosis by reprogramming M1/M2 macrophage polarization | ||
Front Cell Dev Biol Intravenous AAV9 administration results in safe and widespread distribution of transgene in the brain of mini-pig | ||
Thyroid A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE |