验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA and ionomycin stimulated mouse Th2-polarized splenocytes |
| Positive FC detected in | PMA and ionomycin stimulated mouse Th2-polarized splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65074 targets IL-4 in FC (Intra) applications and shows reactivity with Mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Mouse |
| 免疫原 | Partially purified native mouse IL-4 种属同源性预测 |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 4 |
| 别名 | Il 4, Il4, IL-4, interleukin 4 |
| GenBank蛋白编号 | BC027514 |
| 基因名称 | IL-4 |
| Gene ID (NCBI) | 16189 |
| RRID | AB_2934434 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P07750 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IL-4 antibody CL488-65074 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |