CoraLite® Plus 488 Anti-Mouse IL-4 (11B11)
IL-4 Monoclonal Antibody for FC (Intra)
Host / Isotype
Rat / IgG1, kappa
Reactivity
Mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
11B11
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA and ionomycin stimulated mouse Th2-polarized splenocytes |
| Positive FC detected in | PMA and ionomycin stimulated mouse Th2-polarized splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65074-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65074 targets IL-4 in FC (Intra) applications and shows reactivity with Mouse samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | Mouse |
| Immunogen | Partially purified native mouse IL-4 种属同源性预测 |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 4 |
| Synonyms | Il 4, Il4, IL-4, interleukin 4 |
| GenBank Accession Number | BC027514 |
| Gene Symbol | IL-4 |
| Gene ID (NCBI) | 16189 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P07750 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IL-4 antibody CL488-65074 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
