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验证数据展示

  • IF Staining of human tonsillitis using CL488-67051

    IF Staining of human tonsillitis using CL488-67051

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite® Plus 488 IL-4RA/CD124 antibody (CL488-67051, Clone: 1E4C11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Daudi using CL488-67051

    IF Staining of Daudi using CL488-67051

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CoraLite® Plus 488 IL-4RA/CD124 antibody (CL488-67051, Clone: 1E4C11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated IL-4RA/CD124 Monoclonal antibody

IL-4RA/CD124 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-67051

产品说明书

CloneNo. 1E4C11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, IF-P

别名

IL4R, 1E4C11, CD124, IL 4 binding protein, IL 4 receptor subunit alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inDaudi cells
For other applications, we recommend the unconjugated version of this antibody, 67051-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67051 targets IL-4RA/CD124 in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原 IL-4RA/CD124 fusion protein Ag28411 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 4 receptor
别名 IL4R, 1E4C11, CD124, IL 4 binding protein, IL 4 receptor subunit alpha
计算分子量 90 kDa
观测分子量 120 kDa
GenBank蛋白编号NM_000418
基因名称 IL-4R
Gene ID (NCBI) 3566
RRIDAB_2919413
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP24394
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IL-4RA/CD124 antibody CL488-67051Download protocol
Standard Protocols
Click here to view our Standard Protocols