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Anti-Human IL-5 Rabbit Recombinant Antibody, PBS Only

IL-5 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240915D12

Cat no : 98178-1-PBS

Print datasheet

Synonyms

IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I

验证数据展示

  • FC experiment of human PBMCs using 98178-1-RR (same clone as 98178-1-PBS)

    FC experiment of human PBMCs using 98178-1-RR (same clone as 98178-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-5 Rabbit Recombinant Antibody (98178-1-RR, Clone: 240915D12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98178-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98178-1-RR

    Affinity and Kinetic Characterization of 98178-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98178-1-RR against Human IL-5 were performed. The affinity constant is 0.689 nM.

产品信息

98178-1-PBS targets IL-5 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 5 (colony-stimulating factor, eosinophil)
Synonyms IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I
Calculated Molecular Weight 134 aa, 15 kDa
GenBank Accession NumberBC066282
Gene Symbol IL5
Gene ID (NCBI) 3567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05113
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557)