验证数据展示
经过测试的应用
Positive FC (Intra) detected in | PMA, Ionomycin and Brefeldin A treated human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
98178-1-RR targets IL-5 in FC (Intra) applications and shows reactivity with human samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | interleukin 5 (colony-stimulating factor, eosinophil) |
别名 | IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I |
计算分子量 | 134 aa, 15 kDa |
GenBank蛋白编号 | BC066282 |
基因名称 | IL5 |
Gene ID (NCBI) | 3567 |
RRID | AB_3672321 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P05113 |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557)
实验方案
Product Specific Protocols | |
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FC protocol for IL-5 antibody 98178-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |