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验证数据展示

  • FC experiment of human PBMCs using 98178-1-RR

    FC experiment of human PBMCs using 98178-1-RR

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-5 Rabbit Recombinant Antibody (98178-1-RR, Clone: 240915D12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98178-1-RR

    Affinity and Kinetic Characterization of 98178-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98178-1-RR against Human IL-5 were performed. The affinity constant is 0.689 nM.

Anti-Human IL-5 Rabbit Recombinant Antibody

IL-5 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98178-1-RR

Print datasheet

CloneNo. 240915D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98178-1-RR targets IL-5 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 5 (colony-stimulating factor, eosinophil)
Synonyms IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I
Calculated Molecular Weight 134 aa, 15 kDa
GenBank Accession NumberBC066282
Gene Symbol IL5
Gene ID (NCBI) 3567
RRIDAB_3672321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05113
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557)

实验方案

Product Specific Protocols
FC protocol for IL-5 antibody 98178-1-RRDownload protocol
Standard Protocols
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