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IL-6 Recombinant antibody, PBS Only (Capture)

IL-6 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E9

Cat no : 81077-1-PBS

Print datasheet

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

验证数据展示

  • WB analysis of HUVEC using 81077-1-RR (same clone as 81077-1-PBS)

    WB analysis of HUVEC using 81077-1-RR (same clone as 81077-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81077-1-PBS in a different storage buffer formulation.

  • WB analysis of U-87 MG using 81077-1-RR (same clone as 81077-1-PBS)

    WB analysis of U-87 MG using 81077-1-RR (same clone as 81077-1-PBS)

    Protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81077-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80002-1

    Cytometric bead array standard curve of MP80002-1

    Cytometric bead array standard curve of MP80002-1, IL-6 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 81077-1-PBS. Detection antibody: 68659-10-PBS. Standard:HZ-1019 . Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 81077-1-RR

    Affinity and Kinetic Characterization of 81077-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human IL-6 were performed. The affinity constant is below 1 pM.

产品信息

81077-1-PBS targets IL-6 as part of a matched antibody pair:

MP80002-1: 81077-1-PBS capture and 68659-10-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen IL-6 fusion protein Ag10565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 (interferon, beta 2)
Synonyms IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05231
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C