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IL-6 Recombinant antibody

IL-6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E9

Cat no : 81077-1-RR

Print datasheet

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

验证数据展示

  • WB analysis of HUVEC using 81077-1-RR

    WB analysis of HUVEC using 81077-1-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-87 MG using 81077-1-RR

    WB analysis of U-87 MG using 81077-1-RR

    Protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 81077-1-RR

    Affinity and Kinetic Characterization of 81077-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human IL-6 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHUVEC cells, Protein transport inhibitor treated U-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81077-1-RR targets IL-6 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IL-6 fusion protein Ag10565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 (interferon, beta 2)
Synonyms IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
RRIDAB_2918927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05231
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

实验方案

Product Specific Protocols
WB protocol for IL-6 antibody 81077-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols