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Anti-Human IL-6 Rabbit Recombinant Antibody, PBS Only

IL-6 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240670D12

Cat no : 98087-1-PBS

Print datasheet

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

验证数据展示

  • FC experiment of human PBMCs using 98087-1-RR (same clone as 98087-1-PBS)

    FC experiment of human PBMCs using 98087-1-RR (same clone as 98087-1-PBS)

    1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98087-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98087-1-RR (same clone as 98087-1-PBS)

    FC experiment of human PBMCs using 98087-1-RR (same clone as 98087-1-PBS)

    1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98087-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98087-1-RR

    Affinity and Kinetic Characterization of 98087-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98087-1-RR against Human IL-6 were performed. The affinity constant is 0.322 nM.

产品信息

The immunogen of 98087-1-PBS is IL-6 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 (interferon, beta 2)
Synonyms IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2
Calculated Molecular Weight 212 aa, 24 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05231
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.