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验证数据展示

  • FC experiment of human PBMCs using 98087-1-RR

    FC experiment of human PBMCs using 98087-1-RR

    1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

  • FC experiment of human PBMCs using 98087-1-RR

    FC experiment of human PBMCs using 98087-1-RR

    1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

  • Affinity and Kinetic Characterization of 98087-1-RR

    Affinity and Kinetic Characterization of 98087-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98087-1-RR against Human IL-6 were performed. The affinity constant is 0.322 nM.

Anti-Human IL-6 Rabbit Recombinant Antibody

IL-6 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98087-1-RR

产品说明书

CloneNo. 240670D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98087-1-RR targets IL-6 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 6 (interferon, beta 2)
别名 IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2
计算分子量 212 aa, 24 kDa
GenBank蛋白编号BC015511
基因名称 IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
RRIDAB_3672234
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP05231
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

实验方案

Product Specific Protocols
FC protocol for IL-6 antibody 98087-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols