验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
60245-1-Ig targets IL-17F in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | IL-17F fusion protein Ag16310 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | interleukin 17F |
别名 | Cytokine ML 1, IL 17F, IL17F, IL-17F, interleukin 17F, ML 1, ML1 |
计算分子量 | 163 aa, 18 kDa |
观测分子量 | 37 kDa |
GenBank蛋白编号 | BC070124 |
基因名称 | IL-17F |
Gene ID (NCBI) | 112744 |
RRID | AB_2881367 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q96PD4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.
实验方案
Product Specific Protocols | |
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WB protocol for IL-17F antibody 60245-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |