验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
60245-1-Ig targets IL-17F in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | IL-17F fusion protein Ag16310 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 17F |
| Synonyms | Cytokine ML 1, IL 17F, IL17F, IL-17F, interleukin 17F, ML 1, ML1 |
| Calculated Molecular Weight | 163 aa, 18 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC070124 |
| Gene Symbol | IL-17F |
| Gene ID (NCBI) | 112744 |
| RRID | AB_2881367 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96PD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-17F antibody 60245-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |