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验证数据展示

  • WB analysis using 66319-1-Ig

    WB analysis using 66319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66319-1-Ig

    IHC staining of human tonsillitis using 66319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66319-1-Ig

    IHC staining of human tonsillitis using 66319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-21R Monoclonal antibody

IL-21R Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66319-1-Ig

Print datasheet

CloneNo. 1D1C2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

IL21R, IL-21 receptor, IL 21R, IL 21 receptor, CD360

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A172 cells, MJ cells, K-562 cells, TF-1 cells, MOLT-4 cells, HuT 78 cells, NK-92 cells, human placenta tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66319-1-Ig targets IL-21R in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen IL-21R fusion protein Ag21295 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 21 receptor
Synonyms IL21R, IL-21 receptor, IL 21R, IL 21 receptor, CD360
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC004348
Gene Symbol IL-21R
Gene ID (NCBI) 50615
RRIDAB_2881700
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HBE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.

实验方案

Product Specific Protocols
WB protocol for IL-21R antibody 66319-1-IgDownload protocol
IHC protocol for IL-21R antibody 66319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols