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验证数据展示

  • WB analysis using 66319-1-Ig

    WB analysis using 66319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66319-1-Ig

    IHC staining of human tonsillitis using 66319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66319-1-Ig

    IHC staining of human tonsillitis using 66319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-21R Monoclonal antibody

IL-21R Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66319-1-Ig

产品说明书

CloneNo. 1D1C2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

IL21R, IL-21 receptor, IL 21R, IL 21 receptor, CD360

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A172 cells, MJ cells, K-562 cells, TF-1 cells, MOLT-4 cells, HuT 78 cells, NK-92 cells, human placenta tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66319-1-Ig targets IL-21R in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 IL-21R fusion protein Ag21295 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 21 receptor
别名 IL21R, IL-21 receptor, IL 21R, IL 21 receptor, CD360
计算分子量 59 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC004348
基因名称 IL-21R
Gene ID (NCBI) 50615
RRIDAB_2881700
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HBE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.

实验方案

Product Specific Protocols
WB protocol for IL-21R antibody 66319-1-IgDownload protocol
IHC protocol for IL-21R antibody 66319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols