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IL-4 Recombinant antibody, PBS Only (Capture)

IL-4 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240794D7

Cat no : 83894-2-PBS

Print datasheet

Synonyms

IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1

验证数据展示

  • Sandwich ELISA standard curve of MP00829-3

    Sandwich ELISA standard curve of MP00829-3

    Sandwich ELISA standard curve of MP00829-3, Human IL4 Recombinant Matched Antibody Pair - PBS only. 83894-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0104. 83894-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Sample test of MP00829-3

    Sample test of MP00829-3

    Human peripheral blood mononuclear cells (0.5 x 10^6 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 5 μg/mL PHA, and 10 ng/mL recombinant human IL-2. After 7 days of culture, cells were changed into DMEM with 10% fetal bovine serum, 10 ng/mL PMA, 1 μg/mL Ca2+ ionophore, and 5 μg/mL PHA. Cell culture supernate was collected after 1d of stimulation and measured 292.5 pg/mL in stimulated PBMC supernatant, 11.2 pg/mL in unstimulated PBMC supernatant.

  • Sample test of MP00829-3

    Sample test of MP00829-3

    Serum of sixteen individual healthy human donors was measured. The human IL4 concentration of detected samples was determined to be 7.97 ng/mL with a range of 2.25 - 19.49 ng/mL.

  • Cytometric bead array standard curve of MP00829-3

    Cytometric bead array standard curve of MP00829-3

    Cytometric bead array standard curve of MP00829-3, IL-4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83894-2-PBS. Detection antibody: 83894-5-PBS. Standard: Eg0104. Range: 0.625-40 ng/mL.

产品信息

83894-2-PBS targets IL-4 as part of a matched antibody pair:

MP00829-3: 83894-2-PBS capture and 83894-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 4
Synonyms IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1
Calculated Molecular Weight 153 aa, 17 kDa
GenBank Accession NumberBC070123
Gene Symbol IL-4
Gene ID (NCBI) 3565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05112
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C