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CD126/IL-6R alpha Recombinant antibody, PBS Only

CD126/IL-6R alpha Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240734D1

Cat no : 83688-4-PBS

Print datasheet

Synonyms

CD126, IL-6R alpha, 240734D1, IL 6R alpha, IL 6R 1

验证数据展示

  • WB analysis of RAW 264.7 using 83688-4-RR (same clone as 83688-4-PBS)

    WB analysis of RAW 264.7 using 83688-4-RR (same clone as 83688-4-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83688-4-RR (CD126/IL-6R alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83688-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83688-4-RR

    Affinity and Kinetic Characterization of 83688-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83688-4-RR against Human IL-6R were performed. The affinity constant is 2.09 nM.

产品信息

The immunogen of 83688-4-PBS is CD126/IL-6R alpha Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 receptor
Synonyms CD126, IL-6R alpha, 240734D1, IL 6R alpha, IL 6R 1
Calculated Molecular Weight 468 aa, 52 kDa
Observed Molecular Weight65 kDA
GenBank Accession NumberBC132684
Gene Symbol IL-6R alpha
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08887
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).