ILK Monoclonal antibody
ILK Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1E10E4
验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, human placenta tissue, human peripheral blood platelets, rat colon tissue, mouse colon tissue, pig colon tissue, HepG2 cells, HeLa cells, HEK-293 cells, K-562 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IP | See 1 publications below |
产品信息
67724-1-Ig targets ILK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Immunogen | ILK fusion protein Ag21289 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | integrin-linked kinase |
Synonyms | ILK 2, ILK 1, EC:2.7.11.1, Beta-integrin-linked kinase, 59 kDa serine/threonine-protein kinase |
Calculated Molecular Weight | 451 aa, 59 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC001554 |
Gene Symbol | ILK |
Gene ID (NCBI) | 3611 |
RRID | AB_2882910 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q13418 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ILK antibody 67724-1-Ig | Download protocol |
IHC protocol for ILK antibody 67724-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis |