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ILK Monoclonal antibody, PBS Only

ILK Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10E4

Cat no : 67724-1-PBS

Print datasheet

Synonyms

ILK 2, ILK 1, EC:2.7.11.1, Beta-integrin-linked kinase, 59 kDa serine/threonine-protein kinase

验证数据展示

  • WB analysis using 67724-1-Ig (same clone as 67724-1-PBS)

    WB analysis using 67724-1-Ig (same clone as 67724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67724-1-Ig (same clone as 67724-1-PBS)

    IHC staining of human skin cancer using 67724-1-Ig (same clone as 67724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67724-1-Ig (ILK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

产品信息

67724-1-PBS targets ILK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ILK fusion protein Ag21289 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name integrin-linked kinase
Synonyms ILK 2, ILK 1, EC:2.7.11.1, Beta-integrin-linked kinase, 59 kDa serine/threonine-protein kinase
Calculated Molecular Weight 451 aa, 59 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC001554
Gene Symbol ILK
Gene ID (NCBI) 3611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13418
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).