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IMP3 Monoclonal antibody

IMP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E7D6

Cat no : 66247-1-Ig

Print datasheet

Synonyms

MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6

验证数据展示

  • WB analysis of HeLa using 66247-1-Ig

    WB analysis of HeLa using 66247-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66247-1-Ig

    IHC staining of human breast cancer using 66247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 66247-1-Ig

    FC experiment of HepG2 using 66247-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human IMP3 (66247-1-Ig, Clone:1E7D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using 66247-1-Ig

    IF Staining of HepG2 using 66247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 66247-1-Ig

    IF Staining of HepG2 using 66247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 66247-1-Ig

    IF Staining of HepG2 using 66247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SKOV-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66247-1-Ig targets IMP3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen IMP3 fusion protein Ag23602 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Synonyms MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6
Calculated Molecular Weight 184 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC020557
Gene Symbol IMP3
Gene ID (NCBI) 55272
RRIDAB_2881636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NV31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].

实验方案

Product Specific Protocols
WB protocol for IMP3 antibody 66247-1-IgDownload protocol
IHC protocol for IMP3 antibody 66247-1-IgDownload protocol
IF protocol for IMP3 antibody 66247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols