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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of HeLa using 66247-1-Ig (same clone as 66247-1-PBS)

    WB analysis of HeLa using 66247-1-Ig (same clone as 66247-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66247-1-Ig (same clone as 66247-1-PBS)

    IHC staining of human breast cancer using 66247-1-Ig (same clone as 66247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    FC experiment of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human IMP3 (66247-1-Ig, Clone:1E7D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

IMP3 Monoclonal antibody, PBS Only

IMP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66247-1-PBS

产品说明书

CloneNo. 1E7D6

宿主/亚型

Mouse / IgG2b

种属反应性

mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66247-1-PBS targets IMP3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 mouse, human
免疫原 IMP3 fusion protein Ag23602 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
别名 MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6
计算分子量 184 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC020557
基因名称 IMP3
Gene ID (NCBI) 55272
RRIDAB_2881636
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NV31
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].