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IMPDH1 Recombinant antibody

IMPDH1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242329D8

Cat no : 84913-1-RR

Print datasheet

Synonyms

IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1, EC:1.1.1.205

验证数据展示

  • WB analysis of Raji using 84913-1-RR

    WB analysis of Raji using 84913-1-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84913-1-RR

    WB analysis using 84913-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84913-1-RR

    Affinity and Kinetic Characterization of 84913-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84913-1-RR against Human IMPDH1 were performed. The affinity constant is 4.41 nM.

经过测试的应用

Positive WB detected inRaji cells, HeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy IMPDH1 Ready-To-Use IHC Kit. IMPDH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84913-1-RR targets IMPDH1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IMPDH1 fusion protein Ag17473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IMP (inosine monophosphate) dehydrogenase 1
Synonyms IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1, EC:1.1.1.205
Calculated Molecular Weight 563 aa, 60 kDa
Observed Molecular Weight53-60 kDa
GenBank Accession NumberBC033622
Gene Symbol IMPDH1
Gene ID (NCBI) 3614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20839
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa.

实验方案

Product Specific Protocols
WB protocol for IMPDH1 antibody 84913-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols