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IMPDH1 Recombinant antibody, PBS Only (Capture)

IMPDH1 Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242329D8

Cat no : 84913-1-PBS

Print datasheet

Synonyms

IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1, EC:1.1.1.205

验证数据展示

  • WB analysis of Raji using 84913-1-RR (same clone as 84913-1-PBS)

    WB analysis of Raji using 84913-1-RR (same clone as 84913-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

  • WB analysis using 84913-1-RR (same clone as 84913-1-PBS)

    WB analysis using 84913-1-RR (same clone as 84913-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01726-1

    Sandwich ELISA standard curve of MP01726-1

    Sandwich ELISA standard curve of MP01726-1, Human IMPDH1 Recombinant Matched Antibody Pair - PBS only. 84913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17473. 84913-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01726-1

    Sample test of MP01726-1

    The mean IMPDH1 concentration was determined to be 27.79 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load and 45.02 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84913-1-RR

    Affinity and Kinetic Characterization of 84913-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84913-1-RR against Human IMPDH1 were performed. The affinity constant is 4.41 nM.

Planning an IHC experiment? We recommend our IHCeasy IMPDH1 Ready-To-Use IHC Kit. IMPDH1 primary antibody included.

产品信息

84913-1-PBS targets IMPDH1 as part of a matched antibody pair:

MP01726-1: 84913-1-PBS capture and 84913-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen IMPDH1 fusion protein Ag17473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IMP (inosine monophosphate) dehydrogenase 1
Synonyms IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1, EC:1.1.1.205
Calculated Molecular Weight 563 aa, 60 kDa
Observed Molecular Weight53-60 kDa
GenBank Accession NumberBC033622
Gene Symbol IMPDH1
Gene ID (NCBI) 3614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20839
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa.