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IMUP Recombinant antibody

IMUP Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241220A4

Cat no : 84148-7-RR

Print datasheet

Synonyms

Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33

验证数据展示

  • WB analysis using 84148-7-RR

    WB analysis using 84148-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84148-7-RR (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84148-7-RR

    FC experiment of A431 using 84148-7-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug IMUP Recombinant antibody (84148-7-RR, Clone:241220A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, A549 cells, EC109 cells
Positive FC (Intra) detected inA431 cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84148-7-RR targets IMUP in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen IMUP fusion protein Ag22218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 19 open reading frame 33
Synonyms Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33
Calculated Molecular Weight 106 aa, 11 kDa
Observed Molecular Weight20-22 kDa
GenBank Accession NumberBC060319
Gene Symbol IMUP
Gene ID (NCBI) 64073
RRIDAB_3671712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9GZP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

实验方案

Product Specific Protocols
WB protocol for IMUP antibody 84148-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols