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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated INF2 Polyclonal antibody

INF2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20466

Print datasheet

Synonyms

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

验证数据展示

  • IF Staining of HepG2 using CL488-20466

    IF Staining of HepG2 using CL488-20466

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 INF2 antibody (CL488-20466) at dilution of 1:200. DAPI (blue).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 20466-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-20466 is INF2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen INF2 fusion protein Ag14302 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inverted formin, FH2 and WH2 domain containing
Synonyms C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484
Calculated Molecular Weight 1249 aa, 136 kDa
Observed Molecular Weight 180-200 kDa
GenBank Accession NumberBC008756
Gene Symbol INF2
Gene ID (NCBI) 64423
RRIDAB_2919171
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ27J81
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 INF2 antibody CL488-20466Download protocol
Standard Protocols
Click here to view our Standard Protocols